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BIOTECHNOLOGY (Plant tissue culture)

Agar Agar

Agar Agar is an indispensable part of the biotechnology industry. It is used as a gelling agent to prepare semi-solid and solid plant tissue culture medium. The reason why Agar is preferred over other gelling agents are:

 Workable at incubation temperature

Agar Agar when mixed with appropriate portions of water forms into a gel which melts at around 85-95° C and solidifies at 34°C to 41°C.

 Nonreactive with media constituents

Agar-Agar remains unfaced when subjected to media constituents and is immune from plant enzymes.

 Controllable firmness

The firmness of Agar gel depends on the concentration, pH of the medium, and the Agar brand used as culture medium. The Agar concentrations that generally range between 0.5% to 1.0% give the required firmness at a pH specific for plant cell culture media.


Agarose, an Agar Agar derivative is also extensively used for various biotechnological applications:

• For Gel chromatography as a separator of large molecules like antibodies, lipo proteins,etc.
• For DNA and RNA recoveries.
• For live cell imaging, as it improves the quality of images.

Plant Gel (Gellan Gum)

Gellan Gum is used for suspension and plant tissue culture applications.